Optimization of the Sample Preparation Method for DNA Sequencing
نویسندگان
چکیده
منابع مشابه
An automated sample preparation system for large-scale DNA sequencing.
Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA t...
متن کاملCorrection to: Optimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples
Correction Following publication of the original article [1], the authors were alerted by a colleague of a column duplication in Table 1. Since the summary row was correct, though, the interpretation and the conclusion of the article were not affected. The authors have provided the correct table, and would like to acknowledge the assistance of their colleague in the following words: “We thank M...
متن کاملPreparation of PCR products for DNA sequencing.
We demonstrate that routine PCR product analytical agarose gels can also serve as preparative gels for quick DNA template purification before sequencing. The band of interest is excised, placed into a Gel Nebulizer inside a Micropure separator and rapidly purified in a single centrifugation step. Gel-purified PCR product, suitable for manual and automated sequencing, is delivered within 10 min.
متن کاملOptimization and validation of sample preparation for metagenomic sequencing of viruses in clinical samples
BACKGROUND Sequence-specific PCR is the most common approach for virus identification in diagnostic laboratories. However, as specific PCR only detects pre-defined targets, novel virus strains or viruses not included in routine test panels will be missed. Recently, advances in high-throughput sequencing allow for virus-sequence-independent identification of entire virus populations in clinical ...
متن کاملexploring motivation and english test preparation strategies of iranian pre-university candidates during and at the end of test preparation period for konkur examination
the current study aimed at investigating the relationship between motivation and test preparation strategies (tpss) used by iranian pre-university students in their preparation period for the university entrance exam (uee). due to the importance of uee in iran, this study also attempted to show its impact on these two important variables. to this end, 100 pre-university students in an iranian p...
ذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Sciences
سال: 2006
ISSN: 1727-3048
DOI: 10.3923/jbs.2007.194.199